Repetitive DNA sequences—such as Alu and LINE elements—often generate significant background noise in sensitive hybridization assays. Morphix CoT1 DNA is a high-performance blocking reagent engineered to suppress this cross-hybridization. By enriching for rapidly annealing repetitive elements, Morphix CoT1 ensures superior signal-to-noise ratios, allowing for the precise visualization of unique target sequences across FISH, CGH, and NGS workflows.

Morphix CoT1 DNA is optimized for protocols where target specificity is critical. It provides robust suppression of non-specific signals across various genomic platforms:

• Fluorescence In Situ Hybridization (FISH): Suppresses non-specific probe binding to repetitive chromosomal regions, resulting in sharper signals and improved localization.
• Array Comparative Genomic Hybridization (aCGH): Reduces background noise to achieve high-resolution data and minimize false positives in copy number variation (CNV) analysis.
• NGS Target Enrichment: Used during hybridization-capture workflows to block non-specific binding, improving on-target reads and library efficiency.
• General Hybridization Assays: Enhances sensitivity in any protocol involving nucleic acid probes or arrays where genomic DNA is the target.

The CoT fraction of human genomic DNA consists primarily of rapidly annealing, Interspersed Repetitive Sequences (IRS). Morphix CoT1 DNA is prepared from human placental DNA that has been sheared, denatured, and re-annealed under controlled conditions to isolate these repetitive fractions, including the Alu and Kpn families.
When incorporated into a hybridization reaction, Morphix CoT1 functions as a competitive blocker:

1. Competitive Annealing: The enriched repetitive strands in the CoT1 DNA anneal to their complementary sequences (SINEs and LINEs) on the target genomic DNA.
2. Specific Targeting: By effectively occupying these repetitive sites, the reagent prevents labeled probes or primers from binding non-specifically.
3. Signal Optimization: This ensures that hybridization occurs only at unique target sequences, significantly lowering the background signal and increasing data clarity.

• Storage Temperature: We recommend storage at -20°C for long-term preservation.
• Shipping: Due to its exceptional molecualr stability, Morphix CoT1 DNA is shipped at room temperature (RT).
• Durability: The formulation is engineered for maximum resilience; repeated freeze-thaw cycles do not affect product quality or performance.

_{Reference: Weiner, A. M. et al. (1986) Ann. Rev. Biochem. 55, 631–661.}

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